Knowing good and evil for Adam and Eve was as a result of sin not the original plan.
NKJV - 22 Then the LORD God said, "Behold, the man has become like one of Us, to know good and evil. And now, lest he put out his hand and take also of the tree of life, and eat, and live forever" -
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I agree that Adam and Eve sinned by eating from the tree of the knowledge of good and evil, from which God had commanded them not to eat. However, in my opinion, it was their act of eating (that is, disobeying God's command) that constituted the sin, rather than the nature of the tree, or any effect of the fruit itself. To me, this is indicated by the verse cited in the question (Genesis 3:22), in which God Himself said that, once Adam and Eve had eaten of the tree, they had become like God in knowing good and evil. To know what is good and what is evil (as God said that He Himself does, without compromising His holiness) is not the same as choosing and practicing evil (as Adam and Eve did through their disobedience to God's command). I believe that, when we are eternally glorified in God's presence, we will be (as the apostle John said) like Christ (1 John 3:2). We will have free will and know the difference between good and evil (as Christ does), but, like Him, we will never choose evil over good, because our earthly sin nature will have been removed, and our faith will be given its complete and ultimate expression (which I don't think we can even conceive of in this life) in eternal joy, praise, obedience, and service to God.
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