The reason why Abraham decided to split with Lot was to avoid any further conflicts between him and Lot or between his herdsmen and Lot's herdsmen. If the herdsmen went with Lot and were available to be husbands for Lot's daughters, why did the daughters need to sleep with their father to produce offspring?
Genesis 13:8 - 9
ESV - 8 Then Abram said to Lot, "Let there be no strife between you and me, and between your herdsmen and my herdsmen, for we are kinsmen. 9 Is not the whole land before you? Separate yourself from me. If you take the left hand, then I will go to the right, or if you take the right hand, then I will go to the left.
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Since Lot himself lived in Sodom, perhaps the herdsmen for Lot's flocks also lived either in Sodom, or close enough to Sodom (such as in Gomorrah), so that they became caught up in those cities' immoral lifestyle. (In addition, God destroyed not only Sodom and Gomorrah, but "all the valley" in which they were located (Genesis 19:25).) Also, apparently the herdsmen either chose (as Lot's sons-in-law did) not to leave with Lot, or else (even if they had been righteous, and even when combined with Lot, his wife, and their two daughters) did not meet the minimum number of ten righteous people on behalf of whom Abraham had persuaded God to spare the cities (Genesis 18:23-32). In any event, Lot and his two daughters (but not his wife (Genesis 19:26)) were the only survivors. (It is not specifically mentioned that Lot lost his herds, also, but that would seem to be a reasonable assumption.) (Lot is not mentioned again in the Bible after Sodom is destroyed, other than as being the progenitor (by his daughters) of the Moabites and the Ammonites.)
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